Journal: Data in Brief

Article Title: Gene datasets associated with mouse cleft palate

doi: 10.1016/j.dib.2018.03.010

Figure Lengend Snippet: KEGG pathways enriched with a statistically significant number of genes involved in cleft palate.

Article Snippet: Regulation of actin cytoskeleton , Fgf8 Fgfr1 Itgb1 Egfr Itgb6 Pdgfc Sos1 Fgf18 Crk Itgav Fgf10 Rac1 Pdgfrb Fgf9.

Techniques:

Journal: Data in Brief

Article Title: Gene datasets associated with mouse cleft palate

doi: 10.1016/j.dib.2018.03.010

Figure Lengend Snippet: GO biological process terms enriched with a statistically significant number of genes involved in cleft palate.

Article Snippet: Regulation of actin cytoskeleton , Fgf8 Fgfr1 Itgb1 Egfr Itgb6 Pdgfc Sos1 Fgf18 Crk Itgav Fgf10 Rac1 Pdgfrb Fgf9.

Techniques: Cell Differentiation

Journal: Data in Brief

Article Title: Gene datasets associated with mouse cleft palate

doi: 10.1016/j.dib.2018.03.010

Figure Lengend Snippet: GO Molecular Function terms enriched with a statistically significant number of genes involved in cleft palate.

Article Snippet: Regulation of actin cytoskeleton , Fgf8 Fgfr1 Itgb1 Egfr Itgb6 Pdgfc Sos1 Fgf18 Crk Itgav Fgf10 Rac1 Pdgfrb Fgf9.

Techniques: Binding Assay, Protein Binding, Activity Assay, Sequencing

Journal: Data in Brief

Article Title: Gene datasets associated with mouse cleft palate

doi: 10.1016/j.dib.2018.03.010

Figure Lengend Snippet: GO cellular component terms enriched with a statistically significant number of genes involved in cleft palate.

Article Snippet: Regulation of actin cytoskeleton , Fgf8 Fgfr1 Itgb1 Egfr Itgb6 Pdgfc Sos1 Fgf18 Crk Itgav Fgf10 Rac1 Pdgfrb Fgf9.

Techniques:

Journal: International journal of oncology

Article Title: Mast cell chymase promotes angiogenesis and lymphangiogenesis mediated by activation of melanoma inhibitory activity gene family members in oral squamous cell carcinoma.

doi: 10.3892/ijo.2020.4996

Figure Lengend Snippet: Figure 5. Effects of chymase on angiogenesis and lymphangiogenesis in OSCC cells. Effects of chymase and MIA gene family member siRNA transfection on (A) VEGF‑A, (B) VEGF‑C, (C) VEGF‑C and (D) PDGFB secretion in OSCC cells. Changes in the (E and F) adhesive and (G and H) transmigra tory abilities of HSC3 cells to endothelial cells following chymase treatment and/or MIA gene family member siRNA transfection. *P<0.05. OSCC, oral squamous cell carcinoma; MIA, melanoma inhibitory activity; TANGO, transport and Golgi organization protein 1; PDGFB, platelet‑derived growth factor β polypeptide; siRNA, small interfering RNA; VEGF, vascular endo thelial growth factor.

Article Snippet: ELISA kits were used to analyze MIA (cat. no. 11976826001; Roche Diagnostics), MIA2 (cat. no. LS‐F16959; LifeSpan BioSciences, Inc.), TANGO/MIA3 (cat. no. LS‐F52248; LifeSpan BioSciences, Inc.), VEGF‐A (cat. no. RAB0508; Calbiochem; Merck KGaA), VEGF‐C (cat. no. DVEC00; R&D Systems, Inc.), VEGF‐D (cat. no. DVED00; R&D Systems, Inc.) and PDGFB (cat. no. DBB00; R&D Systems, Inc.).

Techniques: Transfection, Adhesive, Activity Assay, Small Interfering RNA

Journal: NPJ Regenerative Medicine

Article Title: Scaffolds with spatiotemporally controlled growth factor delivery and cyclodextrin-enabled antagonism of growth factor receptor sequestration promote cutaneous wound healing

doi: 10.1038/s41536-025-00431-0

Figure Lengend Snippet: a Images of splinted db/db mouse wounds at day 10 post wounding. b Quantification of migrating epithelial tongues from splinted db/db mouse wounds [Error bars correspond to standard deviations from 4 biological specimens with statistical significance assessed using paired 2-way ANOVA followed by Tukey’s multiple comparison testing, * p < 0.05, ** p < 0.01, **** p < 0.0001]. c IHC staining and d quantification of CD31 + cells/mm 2 demonstrating increased number blood vessels with HβCD + EGF treatment [Error bars correspond to standard deviations from four biological specimens with statistical significance assessed using paired student t -test, **** p < 0.0001] (E-epidermis, D-dermis; scale bar = 50 µm). d ELISA demonstrating increased levels of VEGF in splinted db/db mouse wounds upon incorporation of growth factors into HβCD hydrogels [Error bars correspond to standard deviations from four biological specimens with statistical significance assessed using paired 2-way ANOVA followed by Tukey’s multiple comparison testing, * p < 0.05, ** p < 0.01, **** p < 0.0001]. (scale bar = 50 µm).

Article Snippet: For determination of growth factor release from the hydrogels, gelatin hydrogels were loaded onto 0.4 μm PET membranes within a 24 well plate, submerged in PBS, and growth factor release quantified at 0, 12, 24, 48, 96 and 120 h using respective Quantikine ELISA kits (R&D Systems, DEG00 for EGF, DBB00 for PDGF-BB, and DVE00 for VEGF).

Techniques: Comparison, Immunohistochemistry, Enzyme-linked Immunosorbent Assay

Journal: Bone

Article Title: Preservation of Type H Vessels and Osteoblasts by Enhanced Preosteoclast Platelet-Derived Growth Factor type BB Attenuates Glucocorticoid-Induced Osteoporosis in Growing Mice

doi: 10.1016/j.bone.2018.05.025

Figure Lengend Snippet: (A-B) Representative images of immunofluorescence staining of platelet-derived growth factor type BB (PDGF-BB) (green) and tartrate-resistant acid phosphatase (TRAP) (red) with quantification of number of PDGF-BB+ TRAP+ cells (yellow) in femoral primary (A) and secondary spongiosa (B) of 6 week old Ctsk+/+ wild type (WT) and Ctsk−/− mice injected with either vehicle (veh) or prednisolone 10 mg/m2/day (pred) for 4 weeks. DAPI stains nuclei blue. Scale bar, 50 μm. (C) Bone marrow PDGF-BB concentration as analyzed by ELISA. n = 6 per group. Data shown as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: We performed PDGF-BB ELISA analysis of bone marrow supernatant using a Mouse/Rat PDGF-BB Quantikine ELISA kit (R&D Systems) according to the manufacturers’ instructions.

Techniques: Immunofluorescence, Staining, Derivative Assay, Injection, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Expression of PDGF receptors in proliferating and involuting hemangiomas. ( a ) Real-time RT-PCR analysis of PDGFR- α , - β , and PPAR γ in proliferating and involuting hemangiomas (data normalized to 18S rRNA and presented as relative to normal skin; * P <0.05 compared with normal skin, † P <0.05 compared with proliferating hemangioma; n =3). ( b ) Immunostaining of proliferating hemangiomas for PDGFR- α and PDGFR- β (images taken at × 20, inserts show higher magnification; brown=DAB staining, blue=hematoxylin). ( c ) Immunofluorescence double labeling for mesenchymal cell marker α -SMA and PDGFR- β (images taken at × 20, green=PDGFR- β , red= α -SMA)

Article Snippet: The lysates were also used to measure tyrosine phosphorylated PDGF receptors α (PathScan Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit; Cell Signaling Technology, Pickering, Ontario, Canada) and β (PathScan Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit; Cell Signaling Technology).

Techniques: Expressing, Quantitative RT-PCR, Immunostaining, Staining, Immunofluorescence, Labeling, Marker

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Phosphorylated PDGF receptors in proliferating hemangiomas. Immunostaining of proliferating hemangioma specimens for phospho-PDGFR- β ( a ) and phospho-PDGFR- α ( b ) (images taken at × 20, green=PDGFR, blue=DAPI)

Article Snippet: The lysates were also used to measure tyrosine phosphorylated PDGF receptors α (PathScan Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit; Cell Signaling Technology, Pickering, Ontario, Canada) and β (PathScan Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit; Cell Signaling Technology).

Techniques: Immunostaining

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Expression of PDGF signaling axis in hemangioma-derived CD133+ cells. ( a ) Expression of PDGFRs in hemSCs and bm-MPCs (data normalized to 18 S rRNA and presented as relative to bm-MPCs; two different hemSC preparations run in triplicates were averaged). ( b ) Expression of PDGF transcripts in hemSCs (data presented as in ( a ); * P <0.05 compared with bm-MPCs; right panel shows the expression of PDGF-A, PDGF-C, and PDGF-D at a lower scale). ( c ) PDGF-BB levels in media from cells cultured for 48 h in EBM2/20%FBS (with growth factors) (* P <0.05 compared with bm-MPCs). ( d ) PDGF-BB levels in cell lysates determined by ELISA (* P <0.05 compared with bm-MPCs). ( e ) Levels of phosphorylated PDGFR- α and - β in hemSCs with or without exogenous PDGF-BB stimulation (cells were stimulated with 10 ng/ml PDGF-BB; * P <0.05 compared with unstimulated cells). ( f ) Schematic illustration of the hypothesized PDGF polypeptides and the corresponding receptors on hemangioma stem cell surface

Article Snippet: The lysates were also used to measure tyrosine phosphorylated PDGF receptors α (PathScan Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit; Cell Signaling Technology, Pickering, Ontario, Canada) and β (PathScan Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit; Cell Signaling Technology).

Techniques: Expressing, Derivative Assay, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Effect of PDGF-BB on hemSC phenotype and cellular proliferation. ( a ) Fluorescence immunostaining for mesenchymal markers α -SMA, calponin and desmin in hemSCs. Umbilical artery SMCs were used as control for the mural markers. Effect of exogenous PDGF-BB on hemSC migration ( b ) and proliferation ( c ) (data presented as relative to control media treated cells; n =3)

Article Snippet: The lysates were also used to measure tyrosine phosphorylated PDGF receptors α (PathScan Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit; Cell Signaling Technology, Pickering, Ontario, Canada) and β (PathScan Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit; Cell Signaling Technology).

Techniques: Fluorescence, Immunostaining, Control, Migration

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: PDGF-BB inhibits adipogenic differentiation in hemSCs. ( a ) Representative oil red O staining of hemSCs cultured in adipogenic differentiation media supplemented with 10 ng/ml of PDGF-AA, -AB, or -BB. ( b ) Quantitative analysis of adipogenic differentiation in the presence of PDGF polypeptides was assessed by real-time RT-PCR for adipogenesis-specific transcription factors, C/EBP α and PPAR γ (data normalized to 18 S rRNA and presented as relative to adipogenic media; * P <0.05 compared with the adipogenic media; n =3). ( c ) Effect of adipogenic differentiation on PDGFR expression in hemSCs at day 7. ( d ) Oil red O staining of hemSCs cultured with different growth factors (each at 10 ng/ml) illustrating specific inhibition of hemSC adipogenesis by PDGF signaling

Article Snippet: The lysates were also used to measure tyrosine phosphorylated PDGF receptors α (PathScan Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit; Cell Signaling Technology, Pickering, Ontario, Canada) and β (PathScan Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit; Cell Signaling Technology).

Techniques: Staining, Cell Culture, Quantitative RT-PCR, Expressing, Inhibition

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: PDGF-BB, but no other PDGF polypeptides, prevent full functional differentiation of hemSCs. ( a ) Oil red O staining of hemSCs cultured in low (10 ng/ml) and high (50 ng/ml) PDGF polypeptide levels. PDGF-AA and –AB were ineffective against preventing adipogenic differentiation at 10 and 50 ng/ml. ( b ) Quantitative analysis of early adipogenesis-specific transcription factors (C/EBP α and PPAR γ ) and late marker of adipocytes, FABP-4 (* P <0.05 compared to adipogenic media; n =3). ( c ) Expression of FABP-4 in hemSCs treated with 50 ng/ml PDGF-AA. ( d ) Inhibition of adipogenesis by PDGF-BB in bm-MPCs (data presented relative to adipogenic media; * P <0.05 compared with adipogenic media; n =3)

Article Snippet: The lysates were also used to measure tyrosine phosphorylated PDGF receptors α (PathScan Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit; Cell Signaling Technology, Pickering, Ontario, Canada) and β (PathScan Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit; Cell Signaling Technology).

Techniques: Functional Assay, Staining, Cell Culture, Marker, Expressing, Inhibition

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Inhibiting cell-autogenous PDGF signaling enhances adipogenesis. ( a ) Oil red O staining of hemSCs exposed to adipogenic media with or without PDGFR inhibitors, AG-370 and AG-1296, and PDGFR neutralizing antibodies. ( b ) Quantitative analysis of C/EBP α and PPAR γ in cells exposed to PDGF inhibitors as in ( a ) (data presented relative to control media; * P <0.05 compared with control media; † P <0.05 compared with adipogenic media; n =3). ( c ) Effect of PDGFR- α and PDGFR- β- neutralizing antibodies and chemical inhibitors on reversing the inhibition by exogenous PDGF-BB (* P <0.05 compared with PDGF-BB treatment)

Article Snippet: The lysates were also used to measure tyrosine phosphorylated PDGF receptors α (PathScan Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit; Cell Signaling Technology, Pickering, Ontario, Canada) and β (PathScan Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit; Cell Signaling Technology).

Techniques: Staining, Control, Inhibition

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: PDGF-BB employs PDGFR- β in hemSCs. shRNA-mediated knockdown of PDGFR- α ( a ) and PDGFR- β ( b ) in hemSCs at sub-passage 1 (subP1; following 2 weeks of puromycin selection) and sub-passage 6 (six serial passages after puromycin selection) (* P <0.05 compared with control shRNA). ( c ) shRNA-knockdown of PDGFR- α and PDGFR- β in hemSCs reduced the inhibitory effect of PDGF-BB on hemSC adipogenesis (* P <0.05 compared with control shRNA). ( d ) Schematic illustrating the findings of the study and our working hypothesis. High levels of PDGF-BB from hemSCs during proliferation mediate intracellular signaling through PDGFR- β (predominantly PDGFR- ββ homodimer with contribution from PDGFR- αβ heterodimer) to inhibit C/EBP α and PPAR γ expression and adipogenesis (i.e., involution). Upon regression of the vessels and removal of the PDGF-BB source, adipogenesis is triggered

Article Snippet: The lysates were also used to measure tyrosine phosphorylated PDGF receptors α (PathScan Phospho-PDGF Receptor α (Tyr849) Sandwich ELISA Kit; Cell Signaling Technology, Pickering, Ontario, Canada) and β (PathScan Phospho-PDGF Receptor β (Tyr751) Sandwich ELISA Kit; Cell Signaling Technology).

Techniques: shRNA, Knockdown, Selection, Control, Expressing